ecori digested pam2314 plasmid dna (New England Biolabs)
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ecori digested pam2314 plasmid dna
Ecori Digested Pam2314 Plasmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori/EcoRI/pmc13125920-262-9-21
Average 99 stars, based on 15375 article reviews
Ecori Digested Pam2314 Plasmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori/EcoRI/pmc13125920-262-9-21
Average 99 stars, based on 15375 article reviews
ecori digested pam2314 plasmid dna - by Bioz Stars,
2026-09
99/100 stars
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Expressing:Article Title: EBF2 condensates enable adipose thermogenesis through ZFP423 sequestration and epigenetic remodeling. Article Snippet: For transient expression in U2OS cells, full-length mouse EBF2, as well as various truncation and point mutation constructs, were cloned into the LLP340 pEF1α-NLS-scFvGCN4-DNMT3a (mutant) backbone (Addgene plasmid #100944). .. For lentiviral expression, wild-type and mutant EBF2 sequences were subcloned into the pLenti-CMV-NeoR-IRES-ZsGreen1 vector, linearized with Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: PCR products were digested with BamHI (NEB) and NotI (NEB) and ligated into the pCA24N backbone (Addgene, plasmid #87741) using T4 DNA ligase (NEB) with an N-terminal 6xHis under isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression. .. For expression of YfjO in E. coli , yfjO was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Mutagenesis:Article Title: EBF2 condensates enable adipose thermogenesis through ZFP423 sequestration and epigenetic remodeling. Article Snippet: For transient expression in U2OS cells, full-length mouse EBF2, as well as various truncation and point mutation constructs, were cloned into the LLP340 pEF1α-NLS-scFvGCN4-DNMT3a (mutant) backbone (Addgene plasmid #100944). .. For lentiviral expression, wild-type and mutant EBF2 sequences were subcloned into the pLenti-CMV-NeoR-IRES-ZsGreen1 vector, linearized with Plasmid Preparation:Article Title: Loss of Vpr-driven TRAIL-R2 expression protects HIV-infected cells from non-canonical NK cell TRAIL attack Article Snippet: HIV 89.6-ΔVpr was obtained from BEI Resources (Cat.#ARP-13403 - contributed by Dr. Kathleen Collins). .. To make HIV 89.6-ΔVif , the HIV 89.6 plasmid was double digested with ApaI (NEB, Cat.#R0114S) and Article Title: Structural divergence in N-terminal domains of AAA proteases paraplegin (SPG7) and FtsH indicates a key structural function in complex formation Article Snippet: PCR was performed using Taq DNA polymerase (Promega UK Ltd.), purified via gel electrophoresis (1% agarose gel), and the appropriate DNA bands excised and extracted using the gel extraction kit from Qiagen following the manufacturers protocol. .. After restriction digest of amplicon and pRSET_C vector using XhoI and Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: PCR products were digested with BamHI (NEB) and NotI (NEB) and ligated into the pCA24N backbone (Addgene, plasmid #87741) using T4 DNA ligase (NEB) with an N-terminal 6xHis under isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression. .. For expression of YfjO in E. coli , yfjO was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Amplification:Article Title: Structural divergence in N-terminal domains of AAA proteases paraplegin (SPG7) and FtsH indicates a key structural function in complex formation Article Snippet: PCR was performed using Taq DNA polymerase (Promega UK Ltd.), purified via gel electrophoresis (1% agarose gel), and the appropriate DNA bands excised and extracted using the gel extraction kit from Qiagen following the manufacturers protocol. .. After restriction digest of amplicon and pRSET_C vector using XhoI and Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: For construction of B. subtilis add-back (Δ yfjO+yfjO + ) strain, yfjO was cloned into integrative plasmid pBS1C ( ) (Addgene, plasmid #55168) with two recombination sites for insertion at the amyE locus. .. The yfjO coding sequence along with 250 bp flanking region (5’ and 3’) was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: PCR products were digested with BamHI (NEB) and NotI (NEB) and ligated into the pCA24N backbone (Addgene, plasmid #87741) using T4 DNA ligase (NEB) with an N-terminal 6xHis under isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression. .. For expression of YfjO in E. coli , yfjO was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Purification:Article Title: Structural divergence in N-terminal domains of AAA proteases paraplegin (SPG7) and FtsH indicates a key structural function in complex formation Article Snippet: PCR was performed using Taq DNA polymerase (Promega UK Ltd.), purified via gel electrophoresis (1% agarose gel), and the appropriate DNA bands excised and extracted using the gel extraction kit from Qiagen following the manufacturers protocol. .. After restriction digest of amplicon and pRSET_C vector using XhoI and Nucleic Acid Electrophoresis:Article Title: Structural divergence in N-terminal domains of AAA proteases paraplegin (SPG7) and FtsH indicates a key structural function in complex formation Article Snippet: PCR was performed using Taq DNA polymerase (Promega UK Ltd.), purified via gel electrophoresis (1% agarose gel), and the appropriate DNA bands excised and extracted using the gel extraction kit from Qiagen following the manufacturers protocol. .. After restriction digest of amplicon and pRSET_C vector using XhoI and Gel Extraction:Article Title: Structural divergence in N-terminal domains of AAA proteases paraplegin (SPG7) and FtsH indicates a key structural function in complex formation Article Snippet: PCR was performed using Taq DNA polymerase (Promega UK Ltd.), purified via gel electrophoresis (1% agarose gel), and the appropriate DNA bands excised and extracted using the gel extraction kit from Qiagen following the manufacturers protocol. .. After restriction digest of amplicon and pRSET_C vector using XhoI and Sequencing:Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: For construction of B. subtilis add-back (Δ yfjO+yfjO + ) strain, yfjO was cloned into integrative plasmid pBS1C ( ) (Addgene, plasmid #55168) with two recombination sites for insertion at the amyE locus. .. The yfjO coding sequence along with 250 bp flanking region (5’ and 3’) was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Polymerase Chain Reaction:Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: For construction of B. subtilis add-back (Δ yfjO+yfjO + ) strain, yfjO was cloned into integrative plasmid pBS1C ( ) (Addgene, plasmid #55168) with two recombination sites for insertion at the amyE locus. .. The yfjO coding sequence along with 250 bp flanking region (5’ and 3’) was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: PCR products were digested with BamHI (NEB) and NotI (NEB) and ligated into the pCA24N backbone (Addgene, plasmid #87741) using T4 DNA ligase (NEB) with an N-terminal 6xHis under isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression. .. For expression of YfjO in E. coli , yfjO was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Clone Assay:Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: PCR products were digested with BamHI (NEB) and NotI (NEB) and ligated into the pCA24N backbone (Addgene, plasmid #87741) using T4 DNA ligase (NEB) with an N-terminal 6xHis under isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression. .. For expression of YfjO in E. coli , yfjO was PCR amplified from B. subtilis WT 168 genomic DNA, digested with Modification:Article Title: Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in Bacillus subtilis 23S rRNA Article Snippet: PCR products were digested with BamHI (NEB) and NotI (NEB) and ligated into the pCA24N backbone (Addgene, plasmid #87741) using T4 DNA ligase (NEB) with an N-terminal 6xHis under isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression. .. For expression of YfjO in E. coli , yfjO was PCR amplified from B. subtilis WT 168 genomic DNA, digested with |